EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.B9 | expressed in Escherichia coli Rosetta(DE3)plys and BW310 cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.4.B9 | head-to-tail dimer of the A3G catalytic deamination domain A3G-CD2, sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic dehydrate, pH 5.6, 20% (v/v) 2-propanol, 20% (w/v) polyethylene glycol 4000 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.4.B9 | D264A | the variant has 5% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | D370A | the variant has 16% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | F268A | the variant has 25% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | H248G | the variant has 158% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | H250A | the variant has 266% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | P210A | the variant has 1% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | P210G | the variant has 10% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | Q245A | the mutation nearly abolishes the catalytic efficiency to 5% compared to the wild type protein | Homo sapiens |
3.5.4.B9 | Q380A | the variant has 68% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | R256A | the mutation nearly abolishes the catalytic efficiency to 1% compared to the wild type protein | Homo sapiens |
3.5.4.B9 | R374A | the variant has 3% of the catalytic efficiency of the wild type protein | Homo sapiens |
3.5.4.B9 | R376A | the variant has 15% of the catalytic efficiency of the wild type protein | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.B9 | 0.01615 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q380A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.01846 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210G, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.02042 | - |
cytosine in single-stranded viral DNA | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.02252 | - |
cytosine in single-stranded viral DNA | mutant enzyme F268A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.02705 | - |
cytosine in single-stranded viral DNA | mutant enzyme R374A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.03306 | - |
cytosine in single-stranded viral DNA | mutant enzyme H248G, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.03325 | - |
cytosine in single-stranded viral DNA | mutant enzyme H250A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.06323 | - |
cytosine in single-stranded viral DNA | mutant enzyme R376A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.08188 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.1189 | - |
cytosine in single-stranded viral DNA | mutant enzyme D370A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.1639 | - |
cytosine in single-stranded viral DNA | mutant enzyme R256A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.2785 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q245A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.3944 | - |
cytosine in single-stranded viral DNA | mutant enzyme D264A, at pH 7.5 and 37°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.B9 | cytosine in single-stranded viral DNA + H2O | Homo sapiens | - |
uracil in single-stranded viral DNA + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.B9 | Homo sapiens | Q9HC16 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.B9 | Ni-NTA column chromatography and Superdex75 gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.B9 | cytosine in single-stranded viral DNA + H2O | - |
Homo sapiens | uracil in single-stranded viral DNA + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.B9 | A3G | - |
Homo sapiens |
3.5.4.B9 | APOBEC3G | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.B9 | 0.000083 | - |
cytosine in single-stranded viral DNA | mutant enzyme R374A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.000102 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.000165 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210G, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.00017 | - |
cytosine in single-stranded viral DNA | mutant enzyme D370A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0002 | - |
cytosine in single-stranded viral DNA | mutant enzyme R256A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.000517 | - |
cytosine in single-stranded viral DNA | mutant enzyme F268A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.00085 | - |
cytosine in single-stranded viral DNA | mutant enzyme R376A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.001 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q380A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0014 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q245A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0018 | - |
cytosine in single-stranded viral DNA | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.002 | - |
cytosine in single-stranded viral DNA | mutant enzyme D264A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0047 | - |
cytosine in single-stranded viral DNA | mutant enzyme H248G, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.008 | - |
cytosine in single-stranded viral DNA | mutant enzyme H250A, at pH 7.5 and 37°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.B9 | 0.0012 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0012 | - |
cytosine in single-stranded viral DNA | mutant enzyme R256A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0031 | - |
cytosine in single-stranded viral DNA | mutant enzyme R374A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0046 | - |
cytosine in single-stranded viral DNA | mutant enzyme D264A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.005 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q245A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.009 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210G, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0135 | - |
cytosine in single-stranded viral DNA | mutant enzyme R376A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.01425 | - |
cytosine in single-stranded viral DNA | mutant enzyme H248G, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.0143 | - |
cytosine in single-stranded viral DNA | mutant enzyme D370A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.023 | - |
cytosine in single-stranded viral DNA | mutant enzyme F268A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.062 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q380A, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.09 | - |
cytosine in single-stranded viral DNA | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
3.5.4.B9 | 0.24 | - |
cytosine in single-stranded viral DNA | mutant enzyme H250A, at pH 7.5 and 37°C | Homo sapiens |